2023
1. Grieco A, Ruiz-Fresneda MA, Gómez-Mulas A, Pacheco-García JL, Quereda-Moraleda I, Pey AL, Martin-Garcia JM. Structural dynamics at the active site of the cancer-associated flavoenzyme NQO1 probed by chemical modification with PMSF. FEBS Lett. 2023 Nov;597(21):2687-2698. doi: 10.1002/1873-3468.14738
2. El-Araby AM, Jiménez-Faraco E, Feltzer R, Martin-Garcia JM, Karri BR, Ramachandran B, Kim C, Fisher JF, Hermoso JA, Mobashery S. Catalytic process of anhydro-N-acetylmuramic acid kinase from Pseudomonas aeruginosa. J Biol Chem. 2023 Oct;299(10):105198. doi: 10.1016/j.jbc.2023.105198
3.Malla TN, Zielinski K, Aldama L, Bajt S, Feliz D, Hayes B, Hunter M, Kupitz C, Lisova S, Knoska J, Martin-Garcia JM, Mariani V, Pandey S, Poudyal I, Sierra RG, Tolstikova A, Yefanov O, Yoon CH, Ourmazd A, Fromme P, Schwander P, Barty A, Chapman HN, Stojkovic EA, Batyuk A, Boutet S, Phillips GN Jr, Pollack L, Schmidt M. Heterogeneity in M. tuberculosis β-lactamase inhibition by Sulbactam. Nat Commun. 2023 Sep 7;14(1):5507. doi: 10.1038/s41467-023-41246-1
4. Doppler D, Sonker M, Egatz-Gomez A, Grieco A, Zaare S, Jernigan R, Meza-Aguilar JD, Rabbani MT, Manna A, Alvarez RC, Karpos K, Cruz Villarreal J, Nelson G, Yang JH, Carrion J, Morin K, Ketawala GK, Pey AL, Ruiz-Fresneda MA, Pacheco-Garcia JL, Hermoso JA, Nazari R, Sierra R, Hunter MS, Batyuk A, Kupitz CJ, Sublett RE, Lisova S, Mariani V, Boutet S, Fromme R, Grant TD, Botha S, Fromme P, Kirian RA, Martin-Garcia JM, Ros A. Modular droplet injector for sample conservation providing new structural insight for the conformational heterogeneity in the disease-associated NQO1 enzyme. Lab Chip. 2023 Jun 28;23(13):3016-3033. doi: 10.1039/d3lc00176h
5.Jernigan RJ, Logeswaran D, Doppler D, Nagaratnam N, Sonker M, Yang JH, Ketawala G, Martin-Garcia JM, Shelby ML, Grant TD, Mariani V, Tolstikova A, Sheikh MZ, Yung MC, Coleman MA, Zaare S, Kaschner EK, Rabbani MT, Nazari R, Zacks MA, Hayes B, Sierra RG, Hunter MS, Lisova S, Batyuk A, Kupitz C, Boutet S, Hansen DT, Kirian RA, Schmidt M, Fromme R, Frank M, Ros A, Chen JJ, Botha S, Fromme P. Room-temperature structural studies of SARS-CoV-2 protein NendoU with an X-ray free-electron laser. Structure. 2023 Feb 2;31(2):138-151.e5. doi: 10.1016/j.str.2022.12.009
2022
1. Sonker M, Doppler D, Egatz-Gomez A, Zaare S, Rabbani MT, Manna A, Cruz Villarreal J, Nelson G, Ketawala GK, Karpos K, Alvarez RC, Nazari R, Thifault D, Jernigan R, Oberthür D, Han H, Sierra R, Hunter MS, Batyuk A, Kupitz CJ, Sublett RE, Poitevin F, Lisova S, Mariani V, Tolstikova A, Boutet S, Messerschmidt M, Meza-Aguilar JD, Fromme R, Martin-Garcia JM, Botha S, Fromme P, Grant TD, Kirian RA, Ros A. Electrically stimulated droplet injector for reduced sample consumption in serial crystallography. Biophys Rep (N Y). 2022 Sep 29;2(4):100081. doi: 10.1016/j.bpr.2022.100081.
2. Nagaratnam N, Martin-Garcia JM, Yang JH, Goode MR, Ketawala G, Craciunescu FM, Zook JD, Sonowal M, Williams D, Grant TD, Fromme R, Hansen DT, Fromme P. Structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis. PLoS One. 2022 Aug 1;17(8):e0267370. doi: 10.1371/journal.pone.0267370.
3. Zhang S, Hansen DT, Martin-Garcia JM, Zook JD, Pan S, Craciunescu FM, Burnett JC Jr, Fromme P. Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor. Sci Rep. 2022 Jul 12;12(1):11824. doi: 10.1038/s41598-022-15798-z.
4. Martin-Garcia JM. Macromolecular Serial Crystallography (Volume II). Crystals. 2022; 12(6):768. https://doi.org/10.3390/cryst12060768
5. J. M. Martin-Garcia, S. Botha, H. Hu, R. Jernigan, A. Castellví, S. Lisova, F. Gil, B. Calisto, I. Crespo, S. Roy-Chowdhury, A. Grieco, G. Ketawala, U. Weierstall, J. Spence, P. Fromme, N. Zatsepin, D. R. Boer and X. Carpena. Serial macromolecular crystallography at ALBA Synchrotron Light Source. Journal of Synchrotron Rad. (2022) 29 doi:10.1107/S1600577522002508
6. Doppler D, Rabbani MT, Letrun R, Cruz Villarreal J, Kim DH, Gandhi S, Egatz-Gomez A, Sonker M, Chen J, Koua FHM, Yang J, Youssef M, Mazalova V, Bajt S, Shelby ML, Coleman MA, Wiedorn MO, Knoska J, Schön S, Sato T, Hunter MS, Hosseinizadeh A, Kuptiz C, Nazari R, Alvarez RC, Karpos K, Zaare S, Dobson Z, Discianno E, Zhang S, Zook JD, Bielecki J, de Wijn R, Round AR, Vagovic P, Kloos M, Vakili M, Ketawala GK, Stander NE, Olson TL, Morin K, Mondal J, Nguyen J, Meza-Aguilar JD, Kodis G, Vaiana S, Martin-Garcia JM, Mariani V, Schwander P, Schmidt M, Messerschmidt M, Ourmazd A, Zatsepin N, Weierstall U, Bruce BD, Mancuso AP, Grant T, Barty A, Chapman HN, Frank M, Fromme R, Spence JCH, Botha S, Fromme P, Kirian RA, Ros A. Co-flow injection for serial crystallography at X-ray free-electron lasers. J Appl Crystallogr. 2022 Feb 1;55(Pt 1):1-13. doi: 10.1107/S1600576721011079.
2021
1. Pandey S., Calvey G., Katz A.M., Malla T.N., Koua, F.H.M., Martin-Garcia, J.M., Poudyal I., Yang J-H., Vakili M., Yefanov O., Zielinski K.A., Bajt S., Awel S., Doerner K., Frank M., Gelisio L., Jernigan R., Kirkwood H., Kloos M., Koliyadu J., Mariani V., Miller M.D., Mills G., Nelson G., Olmos J.L., Sadri A., Sato T., Tolstikova A., Xu W., Ourmazd A., Spence J.C.H., Schwander P., Barty A., Chapman H.N., Fromme P., Mancuso A.P., Phillips G.N., Bean R., Pollack L., Schmidt M., Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography. IUCrJ, 8, 6, 2021. doi:10.1107/S2052252521008125
2. Martin-Garcia J.M.*. Protein Dynamics and Time Resolved Protein Crystallography at Synchrotron Radiation Sources: Past, Present and Future. Crystals 2021, 11, 521. doi.org/10.3390/cryst11050521
3. Nagaratnam N., Delker S.L., Jernigan R., Edwards T.E., Snider J., Thifault D., Williams D., Nannenga B.L., Stofega M., Sambucetti L., Hsieh J.J., Flint A.J., Fromme P., and Martin-Garcia J.M.*. Structural Insights into the Function of the Catalytically Active Human Taspase1. (Structure 2021, just accepted). doi: 10.1016/j.str.2021.03.008
4. Gisriel C., Fromme P., Martin-Garcia J.M*. Methods for Crystallization and Structural Determination of M-T7 Protein from Myxoma Virus. In: Lucas A.R. (eds) Viruses as Therapeutics. Methods in Molecular Biology. 2021, vol 2225. Humana, New York, NY. doi: 10.1007/978-1-0716-1012-1_8
2020
1. Echelmeier A, Cruz Villarreal J, Messerschmidt M, Kim D, Coe JD, Thifault D, Botha S, Egatz-Gomez A, Gandhi S, Brehm G, Conrad CE, Hansen DT, Madsen C, Bajt S, Meza-Aguilar JD, Oberthür D, Wiedorn MO, Fleckenstein H, Mendez D, Knoška J, Martin-Garcia JM, Hu H, Lisova S, Allahgholi A, Gevorkov Y, Ayyer K, Aplin S, Ginn HM, Graafsma H, Morgan AJ, Greiffenberg D, Klujev A, Laurus T, Poehlsen J, Trunk U, Mezza D, Schmidt B, Kuhn M, Fromme R, Sztuk-Dambietz J, Raab N, Hauf S, Silenzi A, Michelat T, Xu C, Danilevski C, Parenti A, Mekinda L, Weinhausen B, Mills G, Vagovic P, Kim Y, Kirkwood H, Bean R, Bielecki J, Stern S, Giewekemeyer K, Round AR, Schulz J, Dörner K, Grant TD, Mariani V, Barty A, Mancuso AP, Weierstall U, Spence JCH, Chapman HN, Zatsepin N, Fromme P, Kirian RA, Ros A. Segmented flow generator for serial crystallography at the European X-ray free electron laser. Nat Commun. 2020 Sep 9;11(1):4511. doi: 10.1038/s41467-020-18156-7
2. Tang, Y., Saul, J., Nagaratnam, N., Martin-Garcia, J. M., Fromme, P., Qiu, J., & LaBaer, J. Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening. Scientific reports. 2020, 10(1), 13323. doi: 10.1038/s41598-020-70163-2
3. Nagaratnam N, Tang Y, Botha S, Saul J, Li C, Hu H, Zaare S, Hunter M, Lowry D, Weierstall U, Zatsepin N, Spence JCH, Qiu J, LaBaer J, Fromme P, Martin-Garcia JM*. Enhanced X-ray diffraction of in vivo grown μNS crystals by viscous jets at XFELs. Acta Crystallographica section F Struct Biol Communications. 2020 June 1;76(Pt 6):278-289. doi: 10.1107/S2053230X20006172
4. Nass K, Redecke L, Perbandt M, Yefanov O, Klinge M, Koopmann R, Stellato F, Gabdulkhakov A, Schönherr R, Rehders D, Lahey-Rudolph JM, Aquila A, Barty A, Basu S, Doak RB, Duden R, Frank M, Fromme R, Kassemeyer S, Katona G, Kirian R, Liu H, Majoul I, Martin-Garcia JM, Messerschmidt M, Shoeman RL, Weierstall U, Westenhoff S, White TA, Williams GJ, Yoon CH, Zatsepin N, Fromme P, Duszenko M, Chapman HN, Betzel C. In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of genuine co-factors. Nat Commun. 2020 Jan 30;11(1):620. doi: 10.1038/s41467-020-14484-w
5. Fromme P, Graves W, Martin-Garcia JM*. Serial Femtosecond Crystallography: A decade at the forefront of Structural Biology. Encyclopedia of Life Sciences. John Wiley & Sons Ltd, Chichester. 2020 May. doi: 10.1002/9780470015902.a0028964
2019
1. Gisriel C, Coe J, Letrun R, Yefanov OM, Luna-Chavez C, Stander NE, Lisova S, Mariani V, Kuhn M, Aplin S, Grant TD, Dörner K, Sato T, Echelmeier A, Cruz Villarreal J, Hunter MS, Wiedorn MO, Knoska J, Mazalova V, Roy-Chowdhury S, Yang JH, Jones A, Bean R, Bielecki J, Kim Y, Mills G, Weinhausen B, Meza JD, Al-Qudami N, Bajt S, Brehm G, Botha S, Boukhelef D, Brockhauser S, Bruce BD, Coleman MA, Danilevski C, Discianno E, Dobson Z, Fangohr H, Martin-Garcia JM, Gevorkov Y, Hauf S, Hosseinizadeh A, Januschek F, Ketawala GK, Kupitz C, Maia L, Manetti M, Messerschmidt M, Michelat T, Mondal J, Ourmazd A, Previtali G, Sarrou I, Schön S, Schwander P, Shelby ML, Silenzi A, Sztuk-Dambietz J, Szuba J, Turcato M, White TA, Wrona K, Xu C, Abdellatif MH, Zook JD, Spence JCH, Chapman HN, Barty A, Kirian RA, Frank M, Ros A, Schmidt M, Fromme R, Mancuso AP, Fromme P, Zatsepin NA. Membrane protein megahertz crystallography at the European XFEL. Nat Commun. 2019 Nov 4;10(1):5021. doi: 10.1038/s41467-020-14436-4
2. Martin-Garcia JM, Zhu L, Mendez D, Lee MY, Chun E, Li C, Hu H, Subramanian G, Kissick D, Ogata C, Henning R, Ishchenko A, Dobson Z, Zhang S, Weierstall U, Spence JCH, Fromme P, Zatsepin NA, Fischetti RF, Cherezov V, Liu W. High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. IUCrJ. 2019 Apr 5;6(Pt 3):412-425. doi: 10.1107/S205225251900263X
3. Jensen S, Sullivan BT, Hartzler DA, Meza Aguilar J, Awel S, Bajt S, Basu S, Bean R, Chapman H, Conrad C, Frank M, Fromme R, Martin-Garcia JM, Grant TD, Heymann M, Hunter MS, Ketawala G, Kirian RA, Knoska J, Kupitz C, Li X, Liang M, Lisova S, Mariani V, Mazalova VL, Messerschmidt M, Moran M, Nelson G, Oberthuer D, Schaffer A, Sierra RG, Vaughn N, Weierstall U, Wiedorn MO, Xavier PL, Yang JH, Yefanov O, Zatsepin N, Aquila AL, Fromme P, Boutet S, Seidler GT, Pushkar YN. X-ray Emission Spectroscopy at X-ray Free Electron Lasers: Limits to Observation of the Classical Spectroscopic Response for Electronic Structure Analysis. J Phys Chem Lett. 2019 Jan 16. doi: 10.1021/acs.jpclett.8b03595
2018
1. Olmos JL Jr#, Pandey S#, Martin-Garcia JM#, Calvey G, Katz A, Knoska J, Kupitz C, Hunter MS, Liang M, Oberthuer D, Yefanov O, Wiedorn M, Heyman M, Holl M, Pande K, Barty A, Miller MD, Stern S, Roy-Chowdhury S, Coe J, Nagaratnam N, Zook J, Verburgt J, Norwood T, Poudyal I, Xu D, Koglin J, Seaberg M, Zhao Y, Bajt S, Grant T, Mariani V, Nelson G, Subramanian G, Bae E, Fromme R, Fung R, Schwander P, Frank M, White TA, Weierstall U, Zatsepin N, Spence J, Fromme P, Chapman HN, Pollack L, Tremblay L, Ourmazd A, Phillips GN, Schmidt M. Enzyme Intermediates Captured “on-the-fly” by Mix-and-Inject Serial Crystallography. BMC Biology. 2018; 16:59. #These authors contributed equally. doi: 10.1186/s12915-018-0524-5
2. Lan TY, Wierman JL, Tate, Philipp HT, Martin-Garcia JM, Zhu L, Kissick D, Fromme P, Fischetti RF, Liu W, Elser V, Gruner SM. Solving protein structure from sparse serial microcrystal diffraction data at a storage ring synchrotron source. IUCrJ, 2018 Jul 20;5(Pt 5):548-558. doi: 10.1107/S205225251800903X
2017
1. Martin-Garcia JM#, Conrad CE#, Nelson G#, Stander N, Zatsepin NA, Zook J, Zhu L, Geiger J, Chun E, Kissick D, Hilgart MC, Ogata C, Ishchenko A, Nagaratnam N, Roy-Chowdhury S, Coe J, Subramanian G, Schaffer A, James D, Ketwala G, Venugopalan N, Xu S, Corcoran S, Ferguson D, Weierstall U, Spence JCH, Cherezov V, Fromme P, Fischetti RF, Liu W. Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation. IUCrJ. 2017 May 24;4(Pt 4):439-454. #These authors contributed equally. doi: 10.1107/S205225251700570X
2. Deb A, Johnson WA, Kline AP, Scott BJ, Meador LR, Srinivas D, Martin-Garcia JM, Dorner K, Borges CR, Misra R, Hogue BG, Fromme P, Mor TS. Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide. PLoS One. 2017 February 22;12(2): e0172529. doi: 10.1371/journal.pone.0172529
2016
1. Kupitz C, Olmos JL Jr, Holl M, Tremblay L, Pande K, Pandey S, Oberthuer D, Hunter M, Liang M, Aquila A, Tenboer J, Calvey G, Katz A, Chen Y, Wiedorn MO, Knoska J, Meents A, Majriani V, Norwood T, Poudyal I, Grant T, Miller MD, Xu W, Tolstikova A, Morgan A, Metz M, Martin-Garcia JM, Zook JD, Roy-Chowdhury S, Coe J, Nagaratnam N, Meza D, Fromme R, Basu S, Frank M, White T, Barty A, Bajt S, Yefanov O, Chapman HN, Zatsepin N, Nelson G, Weierstall U, Spence J, Schwander P, Pollack L, Fromme P, Ourmazd A, Phillips GN Jr, Schmidt M. Structural enzymology using X-ray free electron lasers. Struct Dyn. 2016 Dec 15;4(4):044003. doi: 10.1063/1.4972069
2. Camara-Artigas A, Ortiz-Salmeron E, Andujar-Sanchez M, Bacarizo J, Martin-Garcia JM. The role of water molecules in the binding of class I and II peptides to the SH3 domain of the Fyn tyrosine kinase. Acta Crystallogr F Struct Biol Commun. 2016 Sep;72(Pt 9):707-12. doi: 10.1107/S2053230X16012310
3. Dörner, K., Martin-Garcia, JM., Kupitz, C., Gong, Z., Mallet, T. C., Chen, L., Wachter, R., Fromme, P. (2016). Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization. Crystal Growth & Design, 16(7), 3838–3845. doi: 10.1021/acs.cgd.6b00384
4. Martin-Garcia JM, Conrad CE, Coe J, Roy-Chowdhury S, Fromme P. Serial femtosecond crystallography: A revolution in structural biology. Arch Biochem Biophys. 2016 July 15;602: 32-47. doi: 10.1016/j.abb.2016.03.036
2015
1. Gong Z, Martin-Garcia JM, Daskalova SM, Craciunescu FM, Song L, Dorner K, Hansen DT, Yang JH, LaBaer J, Hogue BG, Mor TS, Fromme P. Biophysical Characterization of a Vaccine Candidate against HIV-1: The Transmembrane and Membrane Proximal Domains of HIV-1 gp41 as a Maltose Binding Protein Fusion. PLoS One. 2015 Aug 21;10(8): e0136507. doi: 10.1371/journal.pone.0136507
2. Yang JH, Sarrou I, Martin-Garcia JM, Zhang S, Redding KE, Fromme P. Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 October ;114: 1-8. doi: 10.1016/j.pep.2015.05.006
2014
1. Bacarizo J, Martinez-Rodriguez S, Martin-Garcia JM, Andujar-Sanchez M, Ortiz-Salmeron E, Neira JL, Camara-Artigas A. Electrostatic effects in the folding of the SH3 domain of the c-Src tyrosine kinase: pH-dependence in 3D-domain swapping and amyloid formation. PLoS One. 2014 Dec 9;9(12): e113224. doi: 10.1371/journal.pone.0113224
2. Martin-Garcia JM, Hansen DT, Zook J, Loskutov AV, Robida MD, Craciunescu FM, Sykes KF, Wachter RM, Fromme P, Allen JP. Purification and biophysical characterization of the CapA membrane protein FTT0807 from Francisella tularensis. Biochemistry. 2014 Apr 1;53(12):1958-70. doi: 10.1021/bi401644s
3. Camara-Artigas A, Martinez-Rodriguez S, Ortiz-Salmeron E, Martin-Garcia JM. 3D domain swapping in a chimeric c-Src SH3 domain takes place through two hinge loops. J Struct Biol. 2014 Apr;186(1):195-203. doi: 10.1016/j.jsb.2014.02.007
2012
1. Martin-Garcia JM, Ruiz-Sanz J, Luque I. Interfacial water molecules in SH3 interactions: a revised paradigm for polyproline recognition. Biochem J. 2012 Mar 1;442(2):443-51. doi: 10.1042/BJ20111089
2. Camara-Artigas A, Bacarizo J, Andujar-Sanchez M, Ortiz-Salmeron E, Mesa-Valle C, Cuadri C, Martin-Garcia JM, Martinez-Rodriguez S, Mazzuca-Sobczuk T, Ibañez MJ, Allen JP. pH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum. FEBS J. 2012 Oct;279(19):3680-91. doi: 10.1111/j.1742-4658.2012.08730.x
3. Martin-Garcia JM, Luque I, Ruiz-Sanz J, Camara-Artigas A. The promiscuous binding of the Fyn SH3 domain to a peptide from the NS5A protein. Acta Crystallogr D Biol Crystallogr. 2012 Aug;68(Pt 8):1030-40. doi: 10.1107/S0907444912019798
4. De Biasio A, Campos-Olivas R, Sanchez R, Lopez-Alonso JP, Pantoja-Uceda D, Merino N, Villate M, Martin-Garcia JM, Castillo F, Luque I, Blanco FJ. Proliferating cell nuclear antigen (PCNA) interactions in solution studied by NMR. PLoS One. 2012;7(11): e48390. doi: 10.1371/journal.pone.0048390
2010
-
Camara-Artigas A, Andujar-Sanchez M, Ortiz-Salmeron E, Cuadri C, Cobos ES, Martin-Garcia JM. High-resolution structure of an alpha-spectrin SH3-domain mutant with a redesigned hydrophobic core. . 2010 Sep 1;66(Pt 9):1023-7. doi: 10.1107/S1744309110030095
2009
-
Camara-Artigas A, Martin-Garcia JM, Morel B, Ruiz-Sanz J, Luque I. Intertwined dimeric structure for the SH3 domain of the c-Src tyrosine kinase induced by polyethylene glycol binding. . 2009 February 18;583(4):749-53. doi: 10.1016/j.febslet.2009.01.036
2008
-
Palacios A, Munoz IG, Pantoja-Uceda D, Marcaida MJ, Torres D, Martin-Garcia JM, Luque I, Montoya G, Blanco FJ. Molecular basis of histone H3K4me3 recognition by ING4. . 2008 June 6;283(23):15956-64. doi: 10.1074/jbc.M710020200
2007
-
Martin-Garcia JM, Luque I, Mateo PL, Ruiz-Sanz J, Camara-Artigas A. Crystallographic structure of the SH3 domain of the human c-Yes tyrosine kinase: loop flexibility and amyloid aggregation. FEBS Lett. 2007 May 1;581(9):1701-6. Epub 2007 Mar 30. PubMed PMID: 17418139. doi: 10.1016/j.febslet.2007.03.059